CD8+ T cell
Immune-Oncology Assays
- 2D/ 3D Tumour Killing Assay (panel of tumour cells available)
- Antigen-specific CTL-CEFT (phenotype, proliferation, degranulation)
- MART-1 specific CTL- cytotoxicity
- T cell exhaustion
Autoimmunity Assays
- Activation
- Proliferation and function
![](https://www.nexusbioquest.com/app/uploads/2023/02/Group-11285.png)
3D Tumour Killing Models: Tools for screening immune or tumour targeted therapeutics
![](https://www.nexusbioquest.com/app/uploads/2024/02/3D-spheroid-TKA-1-1024x426.png)
![](https://www.nexusbioquest.com/app/uploads/2024/02/TKA-crop-e1708766861767-1024x827.png)
Figure 1: SKOV-3 NLR tumour cells were seeded into 96 well plates, PBMC were added once spheroids were established in the presence of Pembrolizumab or IgG4 control or vehicle (untreated) and imaged every 4 hours for 96 hours using the CellCyte X. Relative spheroid area (%) was measured (A). AUC statistics were calculated using GraphPad Prism v9.5.0
Enzyme-Linked Immuno Spot (ELISpot) is a technique which quantifies immune cells of low abundance which release biomarkers such as cytokines in response to antigenic stimulation. ELISpot is a highly sensitive method to test immune modulators, novel vaccine candidates or de-risk immunogenicity testing in an antigen-specific CD4 and/or CD8 T cell assay.
Condition | Aim |
No Stimulation | Negative control |
PMA | Positive control |
CERI (CMV, EBV, RSV, Influenza) | MHC-I restricted peptide pool to evaluate modulation of CD8+ T-cell memory response |
CPI (CMV, Parainfluenza, Influenza) | Positive protein antigens to evaluate modulation of CD4+ T-cell memory response |
CEF (CMV, EBV, Influenza) | MHC-I restricted peptide pool to evaluate modulation of CD8+ T-cell memory response |
Cyclosporin A (CsA) | Inhibition of immune response |
![](https://www.nexusbioquest.com/app/uploads/2023/04/Screenshot-2023-04-04-at-15.50.37-1024x798.png)
Spot Forming Units (SPU) for IFN-γ per 100,000 PBMC from CERI, CPI and CEF antigens for three donors
![](https://www.nexusbioquest.com/app/uploads/2023/04/Screenshot-2023-04-04-at-15.51.31-1024x385.png)
Evaluation of therapeutic modulation of antigen-specific memory T cell responses to recall antigens
I. T cell response to Tetanus Toxoid, Influenza and PPD antigens
![](https://www.nexusbioquest.com/app/uploads/2023/04/Screenshot-2023-04-05-at-16.30.43.png)
II. Dose response to Influenza antigen
![](https://www.nexusbioquest.com/app/uploads/2023/04/Screenshot-2023-04-05-at-16.30.53.png)
Antigen specific T cells response to a recall antigens. (I) Healthy donors PBMC were stimulated with PHA-M or triple antigen cocktail (Tetanus Toxoid, Influenza and PPD). Cyclosporin was used as a reference treatment. (II) Dose response to Influenza antigen. CD4 and CD8 T cells proliferation was measured by flow cytometry using CTV dilutions.
![](https://www.nexusbioquest.com/app/uploads/2023/03/Micro-1.png)
Drug Discovery Tool
Find the right immune assay for your therapeutic area, modality and target using this interactive Drug Discovery Tool.