Microglia (M) play a role in neuro degeneration by secreting reactive oxygen species (ROS) and pro-inflammatory cytokines, such as Interleukin 1 beta (IL-1β), Interleukin 6 (IL-6) and Tumor Necrosis Factor (TNF-α). Microglial crosstalk with non-neuronal cells shapes their phenotype, either skewing it towards a pro-inflammatory (red arrows) on anti-inflammatory (green arrows) phenotype.

https://doi.org/10.3389/fimmu.2023.1223096

Effect of Dexamethasone on iPSC microglia activation – IL-1β and IL-6.

iPSC derived microglia were stimulated with LPS in the presence or absence of Dexamethasone. Cell culture supernatants were harvested and cytokine levels were quantified (A) IL-1β and (B) IL-6 by TR-FRET and ELISA respectively. Data points show mean +/- SEM of technical replicates.

Other formats: microglia stimulation using LPS+ Nigericin, alpha-synuclein